Scientific References
Balasuriya UB, Heidner HW, Davis NL, Wagner HM, Hullinger PJ, Hedges JF, Williams JC, Johnston RE, David Wilson W,
Liu IK, James MacLachlan N. Alphavirus replicon particles expressing the two major envelope proteins of
equine arteritis virus induce high level protection against challenge with virulent virus in vaccinated horses.
Vaccine 2002; 20:1609-1617.
Baric RS, Yount B, Lindesmith L, Harrington PR, Greene SR, Tseng FC, Davis N, Johnston RE. Klapper DG, Moe CL. Expression and
self-assembly of Norwalk virus capsid protein from Venezuelan equine encephalitis virus replicons. J Virol. 2002; 76(6):3023-30.
Davis NL, Caley IJ, Brown KW, Betts MR, Irlbeck DM, McGrath KM, Connell MJ, Montefiori DC, Frelinger JA, Swanstrom R,
Johnson PR, Johnston RE. Vaccination of macaques against pathogenic simian immunodeficiency virus with Venezuelan equine
encephalitis virus replicon particles. J Virol 2000,74:371-378.
Davis, NL, West A, Reap, E, MacDonald G, Collier M, Dryga, S, Maughan M, Walker, C, McGrath K, Cecil C, Ping LH,
Frelinger J, Olmsted R, Keith P, Swanstrom R, Williamson, C, Johnson P, Montefiori D, Johnston RE. Alphavirus replicon
particles as candidate HIV vaccines. IUBMB Life 2000;53:209-211.
Deming D, Sheahan T, Heise M, Yount B, Davis N, Sims A, Suthar M, Harkema J, Whitmore A, Pickles R, West A, Donaldson E,
Curtis K, Johnston R, Baric R. Vaccine efficacy in senescent mice challenged with recombinant SARS-CoV bearing epidemic
and zoonotic spike variants. PLoS Med. 2006;3:e525.
Durso, RJ., Andjelic, S., Gardner, JP., Margitich, D., Donovan, GP., Arrigale, RR., Wang, X., Maughan, MF., Talarico, TL.,
Olmsted, RA., Heston, WDW., Maddon, PJ., and WC Olson. A Novel Alphavirus Vaccine Encoding Prostate-Specific Membrane
Antigen Elicits Potent Cellular and Humoral Immune Responses. Clin. Cancer Res. 2007. 13:3999-4008.
Eiben G, Velders M, Schreiber H, Cassetti M, Pullen J, Smith L, Kast WM. Establishment of an HLA-A*0201 Human Papillomavirus
Type 16 Tumor Model to Determine the Efficacy of Vaccination Strategies in HLA-A*0201 Transgenic Mice. Cancer Research 2002;
62:5792-5799.
Eralp Y, Wang X, Wang JP, Maughan M, Polo J, Lachman L. Doxorubicin and palitaxel enhance the antitumor efficacy of vaccines
directed against HER 2/neu in a murine mammary carcinoma model. Breast Cancer Research 2004;6(4):R275-83
Elliot, M.B., Chen, T., Terio, NB., Chong, S-Y., Abdullah, R., Luckay, A., Egan, M.A., Boutilier, L.A., Melcille, K., Lerch,
R.A., Long D., Eldridge, J.H., Parks, C.L., Udem, S.A., and G.E. Hancock. Alphavirus replicon particles encoding the fusion
of glycoproteins of respiratory syncytial virus elicit protective immune responses in BALB/c mice and functional serum antibodies
in rhesus macaques. Vaccine. 2007;25:7132-44.
Garcia-Hernandez Mde L, Gray A, Hubby B, Kast WM. In vivo effects of vaccination with six-transmembrane epithelial antigen of
the prostate: a candidate antigen for treating prostate cancer.
Cancer Res. 2007. 67:1344-51.
Goldberg SM, Bartido SM, Gardner JP, Guevara-Patino JA, Montgomery SC, Perales MA, Maughan MF, Dempsey J, Donovan GP,
Olson WC, Houghton AN, Wolchok JD. Comparison of two cancer vaccines targeting tyrosinase: plasmid DNA and recombinant
alphavirus replicon particles. Clinical Cancer Res. 2005;11:8114-21.
Hevey M, Negley D, Pushko P, Smith J, Schmaljohn A. Marburg virus vaccines based upon alphavirus replicons protect
guinea pigs and nonhuman primates. Virology 1998;251:28-37.
Hubby B, Talarico T, Maughan M, Reap EA, Berglund P, Kamrud KI, Copp L, Lewis W, Cecil C, Norberg P, Wagner J,
Watson A, Negri S, Burnett BK, Graham A, Smith JF, Chulay JD. Development and preclinical evaluation of an
alphavirus replicon vaccine for influenza.Vaccine. 2007;25(48):8180-9
Johnston RE, Johnson PR, Connell MJ, Montefiori DC, West A, Collier ML, Cecil C, Swanstrom R, Frelinger JA,
Davis NL. Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon
particle vectors followed by a mucosal challenge with SIVsmE660. Vaccine. 2005;23:4969-79.
Kamrud KI, Custer M, Dudek JM, Owens G, Alterson KD, Lee JS, Groebner JL, Smith JF. Alphavirus replicon approach
to promoterless analysis of IRES elements. Virology. 2007. 360:376-87
Kamrud KI, Alterson KD, Andrews C, Copp LO, Lewis WC, Hubby B, Patel D, Rayner JO, Talarico T, Smith JF.
Analysis of Venezuelan equine encephalitis replicon particles packaged in different coats. PLoS ONE. 2008 Jul
16;3(7):e2709.
Kowalski J, Adkins K, Gangolli S, Ren J, Arendt H, DeStefano J, Obregon J, Tummolo D, Natuk RJ, Brown TP,
Parks CL, Udem SA, and D Long. Evaluation of neurovirulence and biodistribution of Venezuelan equine encephalitis
replicon particles expressing herpes simplex virus type 2 glycoprotein D.
Vaccine. 2007. 25:2296-305
Laust AK, Sur BW, Wang K, Hubby B, Smith JF, Nelson EL. VRP immunotherapy targeting neu: treatment efficacy and
evidence for immunoediting in a stringent rat mammary tumor model. Breast Cancer Res Treat. 2007 Mar 10; [Epub
ahead of print]
Lee JS, Groebner JL, Hadjipanayis AG, Negley DL, Schmaljohn AL, Welkos SL, Smith LA, Smith JF. Multiagent vaccines
vectored by Venezuelan equine encephalitis virus replicon elicits immune responses to Marburg virus and protection
against anthrax and botulinum neurotoxin in mice. Vaccine. 2006;24:6886-92.
Lee JS, Pushko P, Parker MD, Dertzbaugh MT, Smith LA, Smith JF. Candidate vaccine against botulinum neurotoxin
serotype A derived from a Venezuelan equine encephalitis virus vector system. Infect Immune 2001;69:5709-5715.
Lee JS, Dyas BK, Nystrom SS, Lind CM, Smith JF, Ulrich RG. Immune protection against staphylococcal enterotoxin-induced
toxic shock by vaccination with a Venezuelan equine encephalitis virus replicon. J Infect Dis 2002;185:1192-1196.
LoBue AD, Lindesmith L, Yount B, Harrington PR, Thompson JM, Johnston RE, Moe CL, Baric RS. Multivalent norovirus
vaccines induce strong mucosal and systemic blocking antibodies against multiple strains. Vaccine. 2006;24:5220-34.
MacDonald GH, Johnston RE. Role of dendritic cell targeting in Venezuelan equine encephalitis virus pathogenesis.
J Virol 2000; 74:914-922.
Maclachlan NJ, Balasuriya UB, Davis NL, Collier M, Johnston RE, Ferraro GL, Guthrie AJ. Experiences with new
generation vaccines against equine viral arteritis, West Nile disease and African horse sickness. Vaccine. 2007
Jan 16; [Epub ahead of print]
Mok H, Lee S, Utley TJ, Shepherd BE, Polosukhin VV, Collier ML, Davis NL, Johnston RE, Crowe JE Jr. Venezuelan
equine encephalitis virus replicon particles encoding respiratory syncytial virus surface glycoproteins induce
protective mucosal responses in mice and cotton rats. J. Virol. 2007;81:13710-22
Moran TP, Burgents JE, Long B, Ferrer I, Jaffee EM, Tisch RM, Johnston RE, Serody JS. Alphaviral vector-transduced
dendritic cells are successful therapeutic vaccines against neu-overexpressing tumors in wild-type mice.
Vaccine. 2007. 25:6604-12.
Nelson E, Prieto D, Alexander T, Pushko P, Lofts L, Rayner J, Kamrud K, Fralish B, Smith J. Venezuelan equine
encephalitits replicon immunization overcomes intrinsic tolerance and elicits effective anti-tumor immunity to the
'self' tumor-associated antigen, neu in a rat mammary tumor model. Breast Cancer Research and Treatment. 2003;82:169-83.
Nishimoto KP, Laust AK, Wang K, Kamrud KI, Hubby B, Smith JF, and EL Nelson. Restricted and selective tropism of
a Venezuelan equine encephalitis virus-derived replicon vector for human dendritic cells. Viral Immunol. 2007.
20(1):88-104.
Pushko P, Parker M, Ludwig GV, Davis NL, Johnston RE, Smith JF. Replicon-helper systems from attenuated Venezuelan
equine encephalitis virus: expression of heterologous genes in vitro and immunization against heterologous pathogens
in vivo. Virology 1997;239:389-401.
Pushko P, Geisbert J, Parker M, Jahrling P, Smith J. Individual and bivalent vaccines based on alphavirus replicons
protect guinea pigs against infection with Lassa and Ebola viruses. J Virol 2001; 75:11677-11685.
Rayner J, Dryga S, Kamrud K. Alphavirus vectors and vaccination. Reviews in Medical Virology 2002; 12:279-296.
Reap EA, Dryga SA, Morris J, Rivers B, Norberg PK, Olmsted RA, Chulay JD. Cellular and humoral immune responses to
alphavirus replicon vaccines expressing cytomegalovirus pp65, IE1, and gB proteins. Clin Vaccine Immunol. 2007. 14:748-55.
Reap EA, Morris J, Dryga SA, Maughan M, Talarico T, Esch RE, Negri S, Burnett B, Graham A, Olmsted RA, Chulay JD.
Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus. Vaccine.
2007 Oct 16;25(42):7441-9. Epub 2007 Aug 30.
Schleiss MR, Lacayo JC, Belkaid Y, McGregor A, Stroup G, Rayner J, Alterson K, Chulay JD, Smith JF. Preconceptual
administration of an alphavirus replicon UL83 (pp65 homolog) vaccine induces humoral and cellular immunity and
improves pregnancy outcome in the guinea pig model of congenital cytomegalovirus infection. J Infect Dis. 2007;195:789-98.
Schultz-Cherry S, Dybing JK, Davis NL, Williamson C, Suarez DL, Johnston R, Perdue ML. Influenza virus (A/HK/156/97)
hemagglutinin expressed by an alphavirus replicon system protect chickens against a lethal infection with Hong
Kong-origin H5N1 viruses. Virology 2000;278:55-59.
Sylte MJ, Hubby B, Suarez DL. Influenza neuraminidase antibodies provide partial protection for chickens
against high pathogenic avian influenza infection. Vaccine. 2007. 25(19):3763-72.
Thompson JM, Whitmore AC, Konopka JL, Collier ML, Richmond EM, Davis NL, Staats HF, Johnston RE. Mucosal and
systemic adjuvant activity of alphavirus replicon particles.
Proc Natl Acad Sci U S A. 2006. 103:3722-7.
Thornburg NJ, Ray CA, Collier ML, Liao HX, Pickup DJ, Johnston RE. Vaccination with Venezuelan equine encephalitis
replicons encoding cowpox virus structural proteins protects mice from intranasal cowpox virus challenge. Virology.
2007. 362:441-52.
Velders MP, McElhiney S, Cassetti MC, Eiben GL, Higgins T, Kovacs GR, Elmishad AG, Kast WM, Smith LR.
Eradication of established tumors by vaccination with Venezuelan equine encephalitis virus Replicon particles
delivering human papillomavirus 16 E7 RNA. Cancer Res 2001;61:7861-7867.
Wang X, Wang JP, Maughan MF and LB Lachman. Alphavirus replicon particles containing the gene for HER2/neu
inhibit breast cancer growth and tumorigenesis. Breast Cancer Res. 2005;7:R145-R155.
White LJ, Parsons MM, Whitmore AC, Williams BM, de Silva A, Johnston RE. An Immunogenic and Protective
Alphavirus Replicon Particle-Based Dengue Vaccine Overcomes Maternal Antibody Interference in Weanling
Mice. J Virol. 2007 Jul 25; [Epub ahead of print]
Wilson JA, Bray M, Bakken R, Hart MK. Vaccine potential of Ebola virus VP24, VP30, VP35, and VP40 proteins.
Virology 2001;286:384-390.